Regulatory

Part:BBa_K540001

Designed by: Mathilde Dumond   Group: iGEM11_Lyon-INSA-ENS   (2011-09-15)
Revision as of 20:54, 17 September 2011 by Alex273 (Talk | contribs)

rcn, cobalt-sensitive promoter

Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter.

Rcn is also activated by nickel, but we have not characterized this aspect.


Safety

This part is not toxic by itself. However, when using this part, you will probably need to handle cobalt. Cobalt is toxic in all its forms ( ionic or metallic ) by inhalation, ingestion or contact. Wear adapted personal protection equipment ( labcoat, safety glasses, safety gloves ) and dispose of it in appropriate waste containers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor/metal
//promoter
//regulation
Parameters
forward_efficiency
positive_regulators