Coding
Part:BBa_K539714:Design
Designed by: Yang, Yi-Pei Group: iGEM11_NCTU_Formosa (2011-09-20)
kivd (alpha-ketoisovalerate decarboxylase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 578
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In order to design the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region.However, there is one RE site, EcoR1, in kivd. The RE site in kivd has been modified by point mutation.
Source
It is cloned form Lactococcus lactis subsp. lactis KF147