Coding

Part:BBa_K590015

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 05:57, 19 September 2011 by Saushun (Talk | contribs) (→‎Usage and Biology)

sfGFP_mamK_pGA1C3

This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].

Usage and Biology

This part consists of mamK gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. It was made by Gibson Cloning Method.

mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain.

This part was transformed into E.coli and fluorescent filament structures were observed:

MamK sfGFP 3k3-1 uwigem2011part.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1430
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1430
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1430
    Illegal NgoMIV site found at 1156
    Illegal NgoMIV site found at 1523
    Illegal NgoMIV site found at 1670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 80
    Illegal SapI.rc site found at 1802


[edit]
Categories
Parameters
None