Coding
cspB
Part:BBa_K525121:Design
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-11)
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1421
Illegal XhoI site found at 248
Illegal XhoI site found at 866 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1400
Illegal SapI site found at 647
Illegal SapI site found at 859
Illegal SapI site found at 1407
Design Notes
PCR
Source
- PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGTTTAACAACCGTATCCGCAC
- rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]