Device

Part:BBa_K510019

Designed by: Victor Alvarez, Felix Reyes and Yolanda Gonzalez   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Revision as of 21:43, 19 September 2011 by Registry (Talk | contribs)

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Improved Flip Flop (Module I)

Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3130
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4392
    Illegal AgeI site found at 4504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 271


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