Part:BBa_K678063

pJEJAM15

Device consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.

DTU-Denmark-2 2011 Figure 2:

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans with device DIC light.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Front

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Back

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 3747
Illegal XbaI site found at 5303
Illegal XbaI site found at 8047
Illegal PstI site found at 140
Illegal PstI site found at 2657
Illegal PstI site found at 4588
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 910
Illegal PstI site found at 140
Illegal PstI site found at 2657
Illegal PstI site found at 4588
Illegal NotI site found at 5260
Illegal NotI site found at 8086
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 144
Illegal BamHI site found at 3028
Illegal BamHI site found at 4326
Illegal XhoI site found at 700
Illegal XhoI site found at 1082
Illegal XhoI site found at 4186
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 3747
Illegal XbaI site found at 5303
Illegal XbaI site found at 8047
Illegal PstI site found at 140
Illegal PstI site found at 2657
Illegal PstI site found at 4588
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 3747
Illegal XbaI site found at 5303
Illegal XbaI site found at 8047
Illegal PstI site found at 140
Illegal PstI site found at 2657
Illegal PstI site found at 4588
Illegal NgoMIV site found at 3664
Illegal AgeI site found at 1289
Illegal AgeI site found at 1437
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4379
Illegal BsaI.rc site found at 1116
Illegal BsaI.rc site found at 3378
Illegal SapI site found at 322