![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
mdnED
Part:BBa_K627000:Design
Designed by: iGEM2011 Potsdam_Bioware Group: iGEM11_Potsdam_Bioware (2011-09-20)
ABC transporter and N-acetyltransferase from mdn-cluster
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 281
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 777
Illegal SapI.rc site found at 1098
Design Notes
This biobrick was built by PCR using the following PCR primers Forward primer: TAAATGAATTCGCGGCCGCTTCTAGATGCCTCAATATACTACTAAAC Reverse primer: ATTTCTGCAGCGGCCGCTACTAGTATCAGCAAACCCTACTTAATTTC To insert mdnED in the vector pSb1C3, the resulting PCR product and the vector were digested with the restriction enzymes, EcoRI and SpeI.
Source
The BioBrick mdnDE as a part of the microviridin gene (mdn) cluster was isolated from Microcystis aeruginosa strain NIES-843.