Composite

Part:BBa_K546001:Experience

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 20:10, 19 September 2011 by Youri (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K546001

User Reviews

UNIQ405aed045c4cdee2-partinfo-00000000-QINU

No review score entered. Youri van Nuland

Functionality of this part was tested by treating AHL mixtures with strains expressing this part. Liquid cultures were grown overnight, 1 mL aliquots of these cultures were spun down and resuspended in varying concentrations of AHL in PBS (to start the AHL degradation reaction). This was also done with a culture that contains BBa_K546002 (where aiiA is replaced with GFP).

After a 3h incubation, the supernatant was collected and subjected to a reporter strain: BBa_K546002. The result is shown below.

AHL degradation by aiiA reported by BBa K546002.png

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UNIQ405aed045c4cdee2-partinfo-00000002-QINU