Translational_Unit

Part:BBa_K515001

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 13:53, 9 September 2011 by Nikkikapp (Talk | contribs)

IaaH - Indoleacetamide hydrolyase

Indoleacetimide hydrolase catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. The enzyme originates from the plant pathogen Pseudomonas savastanoi which produces the auxin, indole-3-acetic acid via a two step pathway. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_sIndole acetamide
originP. savastanoi
outputindole-3-acetic acid
resistancechloramphenicol