Composite
Part:BBa_K639000
Designed by: Eirik Selsaas Group: iGEM11_NTNU_Trondheim (2011-08-26)
LacI-pLac-mCherry with RBS and terminator
This brick will yield red colonies as it is. When a promoter is inserted in front of the brick, the red color will disappear or be weaker, due to production of LacI that repress lac-promoter. One way of using this brick is to insert a promoter that you suspect, or know is repressed by a molecule or other stimulus. When the promoter is inhibited, transcription of LacI will stop and mCherry will be produced. Thus, the stimuli can be directly measured as intensity of red colour or fluorescense.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1165
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |