Measurement

Part:BBa_K546002:Design

Designed by: M. Hesselman   Group: iGEM11_Wageningen_UR   (2011-09-14)
Revision as of 18:22, 16 September 2011 by PimK (Talk | contribs) (→‎Design Notes)

Lux pL controlled luxR with lux pR controlled GFP(lva tag).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828


Design Notes

The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation, there has been put an (extra) R0063 BioBrick part promoter in front of the F2621 BioBrick device.

Source

The lux p promoters originate from the quorum sensing ability of Vibrio fischeri.

References