Signalling
Part:BBa_K546000:Design
Designed by: M. Hesselman Group: iGEM11_Wageningen_UR (2011-09-14)
Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1821
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Design Notes
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.
Source
The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.