Part:BBa_K590025

The PetroBrick: Strong Constitutive Expression of ADC and AAR in pSB1C3

This part is Synechococcus elongatus PCC7942 Aldehyde Decarbonylase rbs Acyl-ACP Reductase in PSB1C3 high consititutive vector for alkane production

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Use together with Aldehyde Decarbonylase (ADC) to generate alkanes from acyl-ACPs. This part has been codon-optimized for E. coli.

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.

GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 470
  Illegal NheI site found at 493
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1254
  Illegal XhoI site found at 2366
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]