Generator
thRS2-ChZ

Part:BBa_K537002

Designed by: Natasia Kruger   Group: iGEM11_WITS-CSIR_SA   (2011-07-19)
Revision as of 19:11, 19 July 2011 by Registry (Talk | contribs)

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Theophylline Riboswitch 2-CheZ

This DNA part will encode for an RNA riboswitch senstive to theophylline. The riboswitch, in the absence of theophylline, prevents the exposure of the RBS. When theophylline is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement. This riboswitch has a stronger fold increase protein expression compared with Theophylline Riboswitch 1 + RBS + CheZ


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/riboswitch
Parameters
None