Generator
thRS1-ChZ

Part:BBa_K537001:Design

Designed by: Natasia Kruger   Group: iGEM11_WITS-CSIR_SA   (2011-07-19)
Revision as of 16:46, 15 September 2011 by Sashrez (Talk | contribs) (→‎Design Notes)

Theophylline Riboswitch 1-CheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness. The riboswitch and its adjacent CheZ coding region are considered together and should be cloned together in order to overcome the problems previously encountered with the synthetic theophylline riboswitches submitted by teams Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010.

Source

The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch.


References