Generator
atRS-ChZ

Part:BBa_K537000

Designed by: Natasia Kruger   Group: iGEM11_WITS-CSIR_SA   (2011-07-19)
Revision as of 17:26, 19 July 2011 by Registry (Talk | contribs)

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Atrazine Riboswitch-CheZ fusion

This DNA part will encode for an RNA riboswitch senstive to atrazine. The riboswitch, when no atrazine is present, prevents the exposure of the RBS. When atrazine is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rna/riboswitch
Parameters
None