Composite
Part:BBa_K639003
Designed by: Eirik Selsaas Group: iGEM11_NTNU_Trondheim (2011-08-26)
rrnB P1-LacI-pLac-mCherry plausible stress sensor
Biobrick | Part number |
---|---|
LacI with RBS | BBa_J24679 |
Terminator | BBa_B0015 |
pLac | BBa_R0011 |
mCherry with RBS and terminator | BBa_J06702 |
The promotor is a variant of BBa_K112118, that we have made ourselves using PCR, and the PCR product was directly used as insert and connected with the rest of the construct. The final construct was cut with BstBI to verify that the rrnB P1 had been insertet. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see [http://2011.igem.org/Team:NTNU_Trondheim/stress-sensor characterization])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1666
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |