Coding
CspC

Part:BBa_K538004:Design

Designed by: Bas Stringer (Sequence by Paul van Dieken)   Group: iGEM11_Amsterdam   (2011-09-08)
Revision as of 13:20, 15 September 2011 by B.Stringer (Talk | contribs)

CspC (P. irgensii)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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[http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html EMBOSS recoder] detected and silently mutated 1 SapI restriction site. Positions 73 through 79 matched SapI's target sequence: GAAGAGC. The site was recoded by changing the A on position 75 to a G.

Source

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References