Composite
Part:BBa_K568001
Designed by: Team TU Munich 2011 Group: iGEM11_TU_Munich (2011-09-01)
Revision as of 21:07, 14 September 2011 by Thorsten mueller (Talk | contribs)
Optogenetical AND-Gate red/blue light
This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(Ba_K228001). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(BBa_K568000) is induced. Thus, only cells which receive both signals produce the desired substances.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820
Functional Parameters
n/a | Optogenetical AND-Gate red/blue light |
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Categories
Parameters
n/a | Optogenetical AND-Gate red/blue light |