Protein_Domain

Part:BBa_K608406

Designed by: Rüdiger Trojok   Group: iGEM11_Freiburg   (2011-09-14)
Revision as of 13:28, 14 September 2011 by Registry (Talk | contribs)

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Precipitator

Protein domain of the Precipitator. It is an new Leucine Rich Repeat(LRR) with C and N-terminal hagfish domain fragments capping the artifical middle part. This part is one version of three different, designed to bind Nickel by Histidines which are grouped together pointing away from the horseshoe shaped protein. The Precipitator versions differ only in the pattern the position of the Histidine groups. Compare our 3D models on our wiki.

This protein can be used to complex up to 4 Nickel or Cobalt. However the principal design oft he protein is of a particular interest, too. LRR are highly conserved motifs throughout evolution. They appear in all kingdoms of life in almost every thinkable role (Ligases, Receptors, Toxins etc.). Their core is highly conserved and provides a very stable backbone, while the intermediate, non-conserved aminoacids are almost freely interchangeable.

We only submitted one of the three versions, to reduce redundancy in the registry. Please contact us for any questions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None