Protein_Domain
Part:BBa_K525560:Design
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-11)
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1350
Illegal BamHI site found at 2088 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 2341 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 685
Design Notes
Assembled
Source
- BBa_K525499: NADP+ ferredoxin oxidoreductase from Escherichia coli TOP10
- BBa_K123000: bisdA gene from Sphingomonas bisphenolicum AO1, probably synthesized and codon optimized (see experience).
- BBa_K123000: bisdB gene from S. bisphenolicum AO1, probably synthesized and codon optimized (see experience).