Part:BBa_K515102

J23100 promoter - PA2652

Description

Transmembrane chemoreceptor PA2652 coding sequence, with included optimised insulator and RBS (BBa_K515002), expression is under the control of the constitutive promoter J23100 (BBa_J23100). Device is used as an additional chemoreceptor to E. coli endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).<p> Compatibility <p>Device has been tested in E. coli 5α strain Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 710
  Illegal NgoMIV site found at 812
  Illegal AgeI site found at 118
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1019