Regulatory

Part:BBa_K639002:Design

Designed by: Espen Bang Leistad   Group: iGEM11_NTNU_Trondheim   (2011-08-26)
Revision as of 08:21, 26 August 2011 by Registry (Talk | contribs)

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rrnB P1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BioBrick that the Berkeley team made is in BBb format. This means that the BioBrick is flanked by a prefix and a suffix that is different from the ones that are normally used. Therefore, this BioBrick was initially not compatible with the other BioBricks of normal standard (BBa). To deal with this problem, the rrnB P1 promoter was amplified using PCR. The original promoter made by the Berkeley iGEM team was used as template DNA. To make the amplified brick compatible with the rest of our construct, the standard prefix and suffix was added to our primers.


Source

BBa_K112118, rrnB P1 promoter BioBrick in BBb format made by the Berkley iGEM team in 2008.

References