Coding
Part:BBa_K523009
Designed by: Sylvia Ispasanie, Mun Ching Lee Group: iGEM11_Edinburgh (2011-08-04)
Exoglucanase for BioSandwich
This is a modified version of BBa_K118022 for use with the BioSandwich protocol of Edinburgh 2011.
BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 405
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 38
Illegal NgoMIV site found at 411
Illegal NgoMIV site found at 913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 458
Illegal SapI.rc site found at 541
[edit]
Categories
Parameters
None |