Coding

Part:BBa_K523003

Designed by: Mun Ching Lee, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-07-29)
Revision as of 10:04, 29 July 2011 by Registry (Talk | contribs)

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malS for BioSandwich

This is a version of BBa_K523001 but with no RBS and no stop codon.

There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 1505
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None