Translational_Unit

Part:BBa_K523001

Designed by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman   Group: iGEM11_Edinburgh   (2011-07-19)
Revision as of 18:05, 19 July 2011 by Allancrossman (Talk | contribs) (→‎Usage and Biology)

RBS + malS (E. coli periplasmic α-amylase)

This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

The product protein is believed to be periplasmic and thus may not actually be capable of degrading starch, unless it leaks from the periplasm in significant quantities. Its natural function in E. coli presumably involves degrading shorter glucose chains.

The SignalP program predicts that a 17 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None