Translational_Unit

Part:BBa_K523001

Designed by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman   Group: iGEM11_Edinburgh   (2011-07-19)
Revision as of 14:48, 19 July 2011 by Allancrossman (Talk | contribs)

RBS + malS (E. coli periplasmic α-amylase)

This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None