Translational_Unit
Part:BBa_K523001
Designed by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman Group: iGEM11_Edinburgh (2011-07-19)
RBS + malS (E. coli periplasmic α-amylase)
This is the E. coli Amylase gene malS. The part contains the native Ribosome Binding Site.
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1233
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 369
Illegal AgeI site found at 1534 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |