Reporter

Part:BBa_K523000:Design

Designed by: Chris French, Allan Crossman, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-06-28)
Revision as of 21:21, 28 June 2011 by Allancrossman (Talk | contribs) (→‎Source)

Plac + lacZ; with BglII site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the BglII (agatct) site overlaps the BioBrick prefix. Since the prefix ends "ag", only "atct" is included in the BioBrick proper.


Source

Based on part BBa_J33207, which was amplified from E. coli BL21 genomic DNA using primers based on published sequence (Genbank accession J01636, gi:146575).

References