Part:BBa_I20531

CDS for phage k1-5 RNA polymerase

Coding sequence for K1-5 RNA polymerase. Useful for orthogonal transcription. Cognate consensus promoter encoded by BBa_I20530.

Property Rights

All rights not already held by others are PUBLIC DOMAIN as per [http://biobricks.org/bpa/contributions/2/ BPA Contributor Agreement #2] in compliance with [http://rph.stanford.edu/5-1.html Section 5.1.A.3] of the Stanford Research Policy Handbook.

[http://biobricks.org/bpa ] Click to learn about the [http://www.biobricks.org/bpa BPA]

Acknowledgement

[http://www.biosci.utexas.edu/mgm/People/Faculty/profiles/?id=1707 Prof. Ian J. Molineux] of UT Austin suggested the K1-5 RNA polymerase system as a orthogonal transcription system distinct from T3, T7, SP6, et al.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 951
  Illegal SpeI site found at 658
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 658
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1467
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 951
  Illegal SpeI site found at 658
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 951
  Illegal SpeI site found at 658
  
• 1000
  COMPATIBLE WITH RFC[1000]