Coding

Part:BBa_K399001:Design

Designed by: Alyssa Henning and Malinka Walaliyadde   Group: iGEM10_Cornell   (2010-10-26)
Revision as of 01:51, 12 March 2011 by Henninal (Talk | contribs) (→‎Source)

mStrawberry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.

Emission Spectrum

MStrawberry graph.jpg

Characteristic emission spectrum for mStrawberry, showing its percent increase in fluorescence over that of DH5 alhpa cells at specific wavelengths. As expected, peak emission occurs at 600nm.1

Source

mStrawberry was developed by Roger Tsien.1

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572