DNA
Part:BBa_M12203:Design
Designed by: Grant Robinson Group: MIT BE 20.20 - S09 MIT BE 20.20 - S10 (2011-01-07)
Amino Acids 176-240 of E0040
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 119
Design Notes
Not applicable. Forward primer: gtttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac Forward primer: gtttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg Reverse primer: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg
M12203f1: gtttcttcgaattcgcggccgcttctagaggttcaactagcagaccattatcaacaaaatac
M12203f2: gtttcttcgaattcgcggccgcttctagagcaactagcagaccattatcaacaaaatactc
M12203r: gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatgtg
Source
BBa_E0040.