Device

Part:BBa_K392045

Designed by: Tadashi Nakamura, Shuhei Yasumoto, Saya Kakuda, Takahiro Saka   Group: iGEM10_Osaka   (2010-11-07)
Revision as of 21:31, 7 November 2010 by Registry (Talk | contribs)

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endoglucanase secretion testing construct for yeast

Yeast expression device containing C. fermi endoglucanase CenA with engineered secretion tag attached upstream to enable secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1604
    Illegal NotI site found at 2748
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2661
    Illegal BamHI site found at 1797
    Illegal XhoI site found at 2159
    Illegal XhoI site found at 2408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal NgoMIV site found at 1939
    Illegal NgoMIV site found at 2864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830
    Illegal BsaI site found at 1843


[edit]
Categories
Parameters
None