Regulatory

Part:BBa_K112400

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-20)
Revision as of 03:39, 22 October 2008 by ChrisBrown (Talk | contribs)

Promoter for grpE gene - Heat Shock and Ultrasound Sensitive

According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the grpE gene, a known heat shock promoter, is upregulated 24 times when cells are exposed to high frequency ultrasound and microbubble cavitation.


This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma32
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
Parameters
negative_regulators
positive_regulators