Translational_Unit

Part:BBa_K497004

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-24)
Revision as of 05:52, 28 October 2010 by Jo (Talk | contribs)

Agrobacterium tumefaciens bacteriophytochrome & RBS & 3

The Agrobacterium tumefaciens bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. There is a ribosome binding site six bases upstream from the ATG start codon. There is also a 3' transcriptional terminator. Sequence from National Center for Biotechnology.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2369
    Illegal PstI site found at 1664
    Illegal PstI site found at 2060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2369
    Illegal PstI site found at 1664
    Illegal PstI site found at 2060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2369
    Illegal PstI site found at 1664
    Illegal PstI site found at 2060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2369
    Illegal PstI site found at 1664
    Illegal PstI site found at 2060
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1694
    Illegal AgeI site found at 1759
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None