Generator
Cre recomb

Part:BBa_K313008

Designed by: Ryosuke Kamei, Ryo Kariyazono, Shota Suenami, Lena Takayasu, Kentaroh Honda   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 03:16, 28 October 2010 by M.shim (Talk | contribs)

pT7 - Cre recombinase generator

It is the generator of the Cre recombinase.

Cre is transcribed by the T7 RNA polymerase.

By this construct, we conducted recombination experiment along with "Cre recombinase assay device". (Please see here.)

This construct also serves as a positive control of "Cre generator with terminator" series as shown the table below.

Other Cre generator with terminator parts
numberdescriptionSubparts compositonPartsID
1Cre generator with single terminatorpT7-t90%-rbs-cre-dtBBa_K313017
2Cre generator with double terminatorpT7-t90%-t90%-rbs-cre-dtBBa_K313018
3Cre generator with weak single terminatorpT7-t80%-rbs-cre-dtBBa_K313021
4Cre generator with weak double terminatorpT7-t80%-t80%-rbs-cre-dtBBa_K313022

Now we are planning to conduct further research, seeking for appropriate terminator for 4C3 leak-switch.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page and our [http://2010.igem.org/Team:UT-Tokyo/Sudoku_experiments#Terminator_leak wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 117
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None