Part:BBa_K382003

pORE Expression Series Vector with BioBrick MCS (Kan reistance)

Vector for transformation of plants. Cloning in E. coli, transfer to Agrobacterium, integrates into plant genome. Kan resistance marker in bacteria, confers Kan resistance in plants. Has the constitutive promoter pENTCUP2 upstream of multiple cloning site for easy expression of genes in plants. Conforms to Silver BioBrick Assembly Standard 21.

We used this vector for integration of several gene constructs into Arabidopsis.[http://2010.igem.org/Team:Harvard/results]

LTP knock-down amiRNA and ihpRNA (BBa_K382064, BBa_K382070)
Ger3 knock-down ihpRNA (BBa_K382066)
BetVI knock-down ihpRNA (BBa_K382068)
LUT2 knock-down amiRNA (BBa_K382058)
LYC knock-down amiRNA (BBa_K382060)
Beta Ohase I knock-down amiRNA (BBa_K382059)

Plant transformed with this vector:

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 5269
Illegal XbaI site found at 5284
Illegal SpeI site found at 5298
Illegal PstI site found at 5312
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 5269
Illegal SpeI site found at 5298
Illegal PstI site found at 5312
Illegal NotI site found at 5275
Illegal NotI site found at 5305
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 5269
Illegal BglII site found at 5182
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 5269
Illegal suffix found in sequence at 5298
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 5269
Illegal XbaI site found at 5284
Illegal SpeI site found at 5298
Illegal PstI site found at 5312
Illegal NgoMIV site found at 625
Illegal NgoMIV site found at 749
Illegal NgoMIV site found at 2181
Illegal NgoMIV site found at 2772
Illegal NgoMIV site found at 7160
Illegal NgoMIV site found at 7795
Illegal AgeI site found at 341
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 7644
Illegal SapI.rc site found at 7854