Coding

Part:BBa_K382020

Designed by: Patrick Boyle   Group: iGEM10_Harvard   (2010-10-26)
Revision as of 21:14, 4 November 2010 by Pmboyle (Talk | contribs)

Arabidopsis optimized Brazzein

Brazzein is a sweet-tasting protein that is found in the Western African fruit, the Oubli (Pentadiplandra brazzeana). The protein consists of a 54 amino acid sequence. It is sweeter than sugar and is used as an alternative low calorie sweetener.

We characterized[http://2010.igem.org/Team:Harvard/flavor/results] expression of the YFP-tagged version of this part in E. coli. BBa_K382041

YFP tag performance:
Mir BrazzYFP Fig 1-crop.jpeg

Strep tag performance: Brazzein strep western.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/eukaryote/athaliana
Parameters
None