Plasmid

Part:BBa_K382002

Designed by: Mara C. Inniss   Group: iGEM10_Harvard   (2010-10-26)
Revision as of 18:20, 27 October 2010 by Minniss (Talk | contribs)

pORE Expression Series Vector with BioBrick MCS, Basta resistance

Vector for transformation of plants. Cloning in E. Coli, transfer to agrobacteria, integrates in the plant genome. Kan resistance in bacteria, confers Basta resistance in plants. Contains the pENTCUP2 promoter upstream of MCS for constitutive expression of gene of interest.

We used this vector for integration of several gene constructs into Arabidopsis.[http://2010.igem.org/Team:Harvard/results]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5326
    Illegal NheI site found at 4766
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
    Illegal NotI site found at 5341
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5326
    Illegal BglII site found at 5212
    Illegal BglII site found at 7771
    Illegal BamHI site found at 5312
    Illegal XhoI site found at 4790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5326
    Illegal XbaI site found at 5299
    Illegal SpeI site found at 5378
    Illegal PstI site found at 5356
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 749
    Illegal NgoMIV site found at 2181
    Illegal NgoMIV site found at 2772
    Illegal NgoMIV site found at 7220
    Illegal AgeI site found at 341
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7241


[edit]
Categories
//chassis/eukaryote/athaliana
//plasmid
Parameters
None