Coding

Part:BBa_K339003

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-25)
Revision as of 02:06, 30 October 2010 by Emily Hicks (Talk | contribs)

malE31ΔSS

Mutant form of the Maltose binding protein (malE) with signal sequence deleted.


Usage and Biology

This mutant form of the maltose binding protein has the signal sequence deleted (amino acids). This results in the protein not benig localized to the periplasm of the cell, but rather staying in the cytoplasm of the cell. This mutant also has a BamHI site located at codon 32-33 replacing the wild-type sequence Gly-Ile. This two amino acid mutation destroys the folding capabilities of the protein, resulting in a non-folder.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
    Illegal BamHI site found at 96
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


Functional Parameters

[edit]
Categories
Parameters
None