Coding

Part:BBa_K415503

Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 20:55, 28 October 2010 by Ldeming (Talk | contribs)

EYFP_2a_rtta3_ff5 L1L2 MammoBlock Entry Vector

EYFP_2a_rtTa3_FF5 is a construct containing two proteins - EYFP fluorescent protein, and rtTA3 (a DOX-inducible transcription factor that regulates the TRE promoter) seperated by a viral 2A sequence that allows for bicistronic expression of the two genes. FF5 is a micro-RNA controlled degradation tag. This construct is part of the 2010 iGEM mammalian team toggle switch.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 816
    Illegal XbaI site found at 1709
    Illegal PstI site found at 225
    Illegal PstI site found at 1671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1666
    Illegal NheI site found at 804
    Illegal PstI site found at 225
    Illegal PstI site found at 1671
    Illegal NotI site found at 1560
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1666
    Illegal BamHI site found at 1697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 816
    Illegal XbaI site found at 1709
    Illegal PstI site found at 225
    Illegal PstI site found at 1671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1666
    Illegal XbaI site found at 816
    Illegal XbaI site found at 1709
    Illegal PstI site found at 225
    Illegal PstI site found at 1671
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 1433
    Illegal AgeI site found at 8
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None