Part:BBa_K352017
pCooM RBS RFP Double terminator CooA coupled with placI+RBS
This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.
This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.
Agar plates: Our Agar plates with pCooM-RBS-RFP-TT-pLac-RBS-CooA showed that because of low level of constitutive binding of CooA to pCooM promoter, we have observed low level of RFP fluorescence on agar plates.
Culturing CO Sensor E.coli with CO induction
Usage and Biology
We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 647
Illegal AgeI site found at 759
Illegal AgeI site found at 1617 - 1000COMPATIBLE WITH RFC[1000]
//classic/reporter
//function/reporter/fluorescence
| control | specific soret peak @ OD420 |
| emission | 510 nm |
| excitation | 395 nm |
| family | cooA is member of cAMP receptor protein (CRP) and fumavate nitrate reduction (FNR) |
| function | RFP reporter synthesized when carbon monoxide binding cooA protein exists |
| kegg | cooA: avn:Avin_47010 |
| ligands | CO bound cooA |
| n/a | pCooM RBS RFP Double terminator CooA coupled with placI+RBS |
| output | RFP |
| uniprot | cooA: P72322 |