Part:BBa_K313018
terminator leakiness assay device
This is a device that aims to measure the leakiness of a terminator BBa_B1006. If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator. The difference between this part and BBa K313017 is the number of the terminator.
verification experiments
This part is ligated with loxP-gfp-loxP sequence and transformed into E.coli. By incubating E.coli, then extracting plasmids, and sequencing them, it was proved that cre generated by BBa_K313018 is able to catalyze site specific recombination reaction.
Please see our[http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page for detail.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 525
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 211
- 1000COMPATIBLE WITH RFC[1000]
None |