Regulatory
gadA

Part:BBa_K318512:Design

Designed by: Sarah R. Sandock   Group: iGEM10_Wisconsin-Madison   (2010-07-22)
Revision as of 03:30, 28 October 2010 by Pito (Talk | contribs) (→‎References)

gadA (pH promoter and RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

uc


Source

PCR extraction from MG1655 genomic DNA.

References

Tucker, D., et al. Gene Expression Profiling of the pH Response in Escherichia coli. J Bacteriol. 2002. 184(23): 6551–6558.

Waterman, S., et al.Transcriptional Expression of Escherichia coli Glutamate-Dependent Acid Resistance Genes gadA and gadBC in an hns rpoS Mutant. J Bacteriol. 2003. 185(15): 4644-4647.

Castanie-Cornet, M., et al. Control of Acid Resistance in Escherichia coli. J Bacteriol. 1990. 181(11): 3525-3535.

More general information can be found [[http://biocyc.org/ECOLI/NEW-IMAGE?type=OPERON&object=TU586]here]