Composite

Part:BBa_K391007:Design

Designed by: Marianne (Minju) Park   Group: iGEM10_British_Columbia   (2010-10-26)
Revision as of 00:59, 28 October 2010 by Bananas (Talk | contribs) (→‎References)

constitutive promoter-RBS-DspB (J23100:B0034:K391006)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1030
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed.


Source

The gene that encodes our protein, DspB, stems from the bacteria Actinobacillus actinomycetemcomitans.

References

1. Sigma Product Information for 4-Nitrophenyl N-acetyl-β-D-glucosaminide: [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/2/n9376pis.Par.0001.File.tmp/n9376pis.pdf Product Information]