Part:BBa_I10001

Safety Flag

The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.

Invasin

Invasin is a bacterial membrane protein (from Y.pseudotuberculosis) that binds tightly to beta-1-integrins found on the surface of many mamallian cells; interaction between invasin and beta-1-integrins induces the physical uptake of bacteria by mamallian cells via a Rac-1 mediated response (Wong & Isberg, 2005; PMID: 15694850)

Usage and Biology

Chris Anderson & colleagues have demonstrated that the display of invasin on E. coli allows invasin-mediated internalization by mammalian cells; such update does not require additional known adhesion molecules. Their work was done in E. coli MC1061, a fim+ strain, and CAMC600, a fim- strain (Andereson et al., 2005 PMID: 16330045). Inv+ E. coli can invade a broad range of tumor cells (epithelial, hepatocarcinoma, and osteosarcoma).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 840
  Illegal AgeI site found at 349
  Illegal AgeI site found at 673
  Illegal AgeI site found at 808
  Illegal AgeI site found at 886
  Illegal AgeI site found at 2395
  Illegal AgeI site found at 2899
  
• 1000
  COMPATIBLE WITH RFC[1000]