Generator

Part:BBa_K395701

Designed by: Yumiko Kinoshita   Group: iGEM10_Tokyo_Tech   (2010-10-04)
Revision as of 23:37, 27 October 2010 by Taichi (Talk | contribs)

rbs+crtZ under plac promoter for double plasmids

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon. This part applies to ③ and ⑤

Fig.2 zeaxanthin pellet and plate, TLC
These works are done by Yumiko Kinoshita


These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None