Tag
Part:BBa_K496003
Designed by: Shunichi MAKINO Group: iGEM10_HokkaidoU_Japan (2010-10-17)
RBS + SlrP secretion tag, salmonella T3 sec. sys.
Leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins as GFP.
See BBa_K496004 for attempts to use this part to inject GFP to eukaryotic cell.
References
- Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |