Regulatory
Curli

Part:BBa_K342000:Design

Designed by: Philippe Thomas   Group: iGEM10_INSA-Lyon   (2010-10-24)
Revision as of 23:25, 27 October 2010 by Philou (Talk | contribs) (→‎Source)

Curli Promoter induced by temperature, shaking speed and osmolarity,


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to only synthesize the promoter with the site of regulation by csgD. After analyzing the sequence, we decided to begin our promoter 20bp before the csgD box, and to finish it on the +1bp of transcription. It corresponds to 71 bp.
We added a non-CDS (non coding sequence) prefix and suffix to our sequence, to fit with iGEM standards.
Need the part BBa_K342003 to be functional.

Source

To design this sequence, we based our work on a table of compared sequences of the intergenic region csgA-csgD realized on various strains modificated, with the sauvage strain PHL818(MG1655 ompR234) as reference.
This study had been realized by the unit of Microbiology and Genetic (CNRS UMR 5122), Institut National des Sciences Appliquees,Lyon.

References