Regulatory

Part:BBa_K354000:Design

Designed by: Gregory Meyer   Group: iGEM10_WITS-South_Africa   (2010-10-19)
Revision as of 21:25, 27 October 2010 by Chelle (Talk | contribs) (→‎References)

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Lac/Ara-1 IPTG Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The affiliation between AraC and its binding sequence had to be ensured so as to prevent basal expression and false triggering of the promoter.


Source

The promoter was synthesised via primer extension.

References

[1] Lutz R. & Bujard H. (1997), Independent and tight regulation of transcriptional units in Escherchia coli via the LacR/O, the TetR/O and AraC/I_1- I_2 regulatory elements. Nucleic Acids Research 25: 1203-1210.

[2] Schleif R. (2000), Regulation of the L-arabinose operon in Escherichia coli. Trends in Genetics 16: 559-565.

[3] Hansen L.S., Knudsen S. & Sorensen S.J. (1998), The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studies in Escherichia coli and Pseudomonas fluorescens. Current Microbiology 36: 341-347.

[4] Marco Weinberg, PhD. Antiviral Gene Therapy Research Unit, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, 7 York Rd. Parktown 2193 SOUTH AFRICA, marc.weinberg@wits.ac.za.